Rhodopsin and bacteriorhodopsin: structure-function relationships
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چکیده
منابع مشابه
Photoaffinity labeling of rhodopsin and bacteriorhodopsin.
Photoaffinity labeling with bovine rhodopsin using a retinal with a fixed 11-cis-ene cross-linked exclusively to Trp-265/Leu-266 in helix F, showing that the beta-ionone C-3 is close to helix F. Moreover, since these labeled amino acids are in the middle of helix F, while the Schiff-base linkage to Lys-296 at the other terminus of the chromophore is also in the middle of helix G, the chromophor...
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Molecular interactions and mechanical properties that contribute to the stability and function of proteins are complex and of fundamental importance. In this study, we used single-molecule dynamic force spectroscopy (DFS) to explore the interactions and the unfolding energy landscape of bovine rhodopsin and bacteriorhodopsin. An analysis of the experimental data enabled the extraction of parame...
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II. Structure and function of rhodopsin and bacteriorhodopsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 A. Organization of the proteins in the membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 296 B. The photobleaching sequence of rhodopsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 C. The photocycle of bact...
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EPR spectroscopy in combination with site directed spin labeling (SDSL) has become a valuable tool for structural investigations as well as for kinetic studies on proteins. This method has been especially useful for membrane proteins in yielding structural and functional data. This information is not easily available from other techniques, like, e.g., X-ray crystallography or electron microscop...
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In order to delineate functionally important domains in erythropoietin (Epo), we have prepared and tested a series of amino acid replacements at 51 conserved sites predicted to be on the surface of the molecule. Alanine replacements permitted preservation of a-helical structure. Wild type and mutant Epo cDNAs were transiently expressed at high levels in COS1 and COS7 cells. The biological activ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80805-3